Biology exam trends & analysis (2019–2025)
Across 2019-2025 the four modules are examined remarkably evenly (Module 6 Genetic Change leads with 199 marks, followed by Module 8 Non-infectious Disease (177), Module 7 Infectious Disease (163) and Module 5 Heredity (161)). By topic, meiosis and reproduction is by far the most examined dot point, then homeostasis/feedback and epidemiology; immune response, biotechnology applications, mutation types and mutation-and-evolution also recur in every paper.
Based on 303 questions across 7 official NESA exam papers, their marking guidelines and marking feedback.
Work in progress
These exam-trend insights are an early release. The frequencies, mark ranges and figures are still being verified against the official NESA past papers and may change. Treat them as a study guide, not a guarantee of what will be examined.
Most-examined dot points
By module
Every dot point, by exam frequency
Click any dot point for the full verbatim syllabus wording, worked answers and past questions.
Showing 34 of 34 dot points
| Dot point | Times | Marks | Years | Most common mistake |
|---|---|---|---|---|
| Meiosis and gamete formation explainedM5 Give specific detail on meiosis and fertilisation, not general info; know meiosis (not mitosis) produces gametes | 36× | 1–6 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Give specific detail on meiosis and fertilisation, not general info; know meiosis (not mitosis) produces gametes |
| Homeostasis, feedback, thermoregulation and osmoregulationM8 Use precise terms (vasodilation, hypothalamus, evaporative cooling) not general descriptions; understand the hypothalamus detects internal temperature and sweat evaporation cools the skin | 23× | 1–6 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Use precise terms (vasodilation, hypothalamus, evaporative cooling) not general descriptions; understand the hypothalamus detects internal temperature and sweat evaporation cools the skin |
| Epidemiology: incidence, prevalence, mortality and study designsM8 Discuss data beyond repeating the table, identify dominant/recessive alleles, and write clearly and succinctly | 18× | 1–7 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Discuss data beyond repeating the table, identify dominant/recessive alleles, and write clearly and succinctly |
| Biotechnology applications in agriculture, medicine and industryM6 Show cause and effect; use quantitative data; link the benefit to aquaculture | 16× | 1–7 | 2020, 2021, 2022, 2023, 2024, 2025 | Show cause and effect; use quantitative data; link the benefit to aquaculture |
| Types of mutation: point, silent, frameshift and chromosomalM6 Present information concisely, interpret the table requirements, and understand each mutation type's effect on offspring | 16× | 1–4 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Present information concisely, interpret the table requirements, and understand each mutation type's effect on offspring |
| Local, regional and global strategies to limit disease spreadM7 Interpret different data types to identify trends, link trends to the data, and use terms like incidence/distribution correctly | 14× | 1–7 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Interpret different data types to identify trends, link trends to the data, and use terms like incidence/distribution correctly |
| Mutation, gamete variation and the source of new allelesM6 Use specific DNA and evolution detail; refer to natural selection, not Lamarckian inheritance | 14× | 1–7 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Use specific DNA and evolution detail; refer to natural selection, not Lamarckian inheritance |
| Antivirals, antibiotics, resistance and immunisationM7 Mismatched drug to pathogen (antibiotics on viruses); confused antibiotics with antibodies; said the body, not bacteria, develops resistance | 13× | 1–7 | 2019, 2020, 2021, 2022, 2023, 2025 | Mismatched drug to pathogen (antibiotics on viruses); confused antibiotics with antibodies; said the body, not bacteria, develops resistance |
| Technologies for hearing and vision disordersM8 Provide a clear key per ear; read the dB axis; plot precisely | 13× | 1–5 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Provide a clear key per ear; read the dB axis; plot precisely |
| Transcription and translation explainedM5 Demonstrate clear understanding of both transcription and translation | 13× | 1–5 | 2019, 2020, 2021, 2022, 2023, 2024 | Demonstrate clear understanding of both transcription and translation |
| Causes of infectious disease and pathogen typesM7 Lacked specificity (single-stranded RNA, not just single-stranded); classified by processes not structures | 12× | 1–3 | 2020, 2021, 2022, 2023, 2024, 2025 | Lacked specificity (single-stranded RNA, not just single-stranded); classified by processes not structures |
| Adaptive immune response, humoral and cell-mediated immunityM7 Just described the graphs without applying syllabus knowledge of vaccination, immunity and antibody action | 11× | 1–8 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Just described the graphs without applying syllabus knowledge of vaccination, immunity and antibody action |
| Effects of mutation on amino acid sequence: coding vs non-coding DNAM6 Use the stimulus and link the mutation to a changed structure then loss of cell-division control; understand uncontrolled division causes cancer | 11× | 1–4 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Use the stimulus and link the mutation to a changed structure then loss of cell-division control; understand uncontrolled division causes cancer |
| Recombinant DNA, CRISPR, whole genome sequencing and gene therapyM6 Used general statements grouping diseases/technologies; lacked genetic-level detail; confused genetic technology with biotechnology | 10× | 1–7 | 2019, 2020, 2021, 2023, 2024, 2025 | Used general statements grouping diseases/technologies; lacked genetic-level detail; confused genetic technology with biotechnology |
| DNA replication explainedM5 Understand replication yields two semiconservative molecules, each with one parent strand | 9× | 1–3 | 2019, 2020, 2021, 2022, 2023, 2024, 2025 | Understand replication yields two semiconservative molecules, each with one parent strand |
| Codominance, incomplete dominance and multiple alleles explainedM5 Provide an appropriate key to aid interpretation of the Punnett squares | 7× | 1–5 | 2019, 2020, 2021, 2022, 2023, 2025 | Provide an appropriate key to aid interpretation of the Punnett squares |
| Pedigree analysis for mutationsM6 Make a definitive statement; a single band means homozygous; not probability/Punnett | 7× | 1–3 | 2020, 2021, 2022, 2023, 2024, 2025 | Make a definitive statement; a single band means homozygous; not probability/Punnett |
| Disease management: pharmaceuticals, gene therapy, lifestyleM8 Did not use the full grid with an appropriate, evenly divided scale | 6× | 1–3 | 2020, 2021, 2022, 2025 | Did not use the full grid with an appropriate, evenly divided scale |
| Koch and Pasteur, and Koch's postulatesM7 Apply biological knowledge to the situation using flowcharts | 6× | 1–5 | 2021, 2022, 2024 | Apply biological knowledge to the situation using flowcharts |
| Punnett squares and Mendelian inheritanceM5 Distinguish allele from genotype and use all stem information to determine allele combinations | 6× | 1–3 | 2019, 2020, 2021, 2022, 2023, 2024 | Distinguish allele from genotype and use all stem information to determine allele combinations |
| Causes of non-infectious diseaseM8 Specifically define disease categories (not just 'not caused by a pathogen') and use data and examples from all parts of Q33 to support the judgement | 5× | 1–8 | 2019, 2020, 2022 | Specifically define disease categories (not just 'not caused by a pathogen') and use data and examples from all parts of Q33 to support the judgement |
| Innate immune response in animals, first and second lines of defenceM7 Lacked precise terminology such as 'engulf' or 'enclose' | 5× | 1–2 | 2019, 2022, 2023, 2025 | Lacked precise terminology such as 'engulf' or 'enclose' |
| DNA structure: Watson, Crick, Franklin and WilkinsM5 Answer about DNA/chromosome structure, not location or general cell differences | 4× | 1–3 | 2020, 2022, 2024 | Answer about DNA/chromosome structure, not location or general cell differences |
| Effects of biotechnology on biodiversityM6 Use terminology specific to biodiversity and biotechnology and give specific examples rather than general comments | 4× | 2–9 | 2019, 2021, 2022, 2023 | Use terminology specific to biodiversity and biotechnology and give specific examples rather than general comments |
| Modes of disease transmissionM7 Address the plural 'diseases' by giving more than one disease example | 4× | 1–3 | 2019, 2022, 2024, 2025 | Address the plural 'diseases' by giving more than one disease example |
| Prevention of non-infectious diseaseM8 Use specific real educational programs as examples (more than one), not screening programs, genetic testing or legislation | 4× | 3–6 | 2019, 2022, 2024, 2025 | Use specific real educational programs as examples (more than one), not screening programs, genetic testing or legislation |
| Sex-linked inheritance explainedM5 Poor use of formats (Punnett squares); limited understanding of autosomal versus sex-linked inheritance | 4× | 1–4 | 2019, 2020, 2021, 2025 | Poor use of formats (Punnett squares); limited understanding of autosomal versus sex-linked inheritance |
| Causes of mutation: physical, chemical and biological mutagensM6 Did not distinguish gametic from somatic mutations; unclear which radiation causes DNA damage | 3× | 1–2 | 2021, 2024, 2025 | Did not distinguish gametic from somatic mutations; unclear which radiation causes DNA damage |
| Nutritional and environmental diseasesM8 Named a pathogen/genetic disease instead of environmental; gave a symptom not a cause | 3× | 1–2 | 2021, 2023, 2024 | Named a pathogen/genetic disease instead of environmental; gave a symptom not a cause |
| Pathogen adaptations for entry and transmissionM7 Name a specific pathogen (not a disease) and distinguish entry adaptations from immune-evasion or transmission adaptations | 3× | 2 | 2019, 2020, 2025 | Name a specific pathogen (not a disease) and distinguish entry adaptations from immune-evasion or transmission adaptations |
| Plant responses to pathogens, physical and chemical defencesM7 Failed to name a plant disease; stated 'has an impact' without a directional effect on agriculture | 3× | 1–4 | 2023, 2024 | Failed to name a plant disease; stated 'has an impact' without a directional effect on agriculture |
| Aboriginal protocols and the development of medicinesM7 | — | — | not yet | — |
| Future directions of genetic research: germline editing, gene drives and synthetic biologyM6 | — | — | not yet | — |
| Genetic disorders: cystic fibrosis, sickle cell, Huntington'sM8 | — | — | not yet | — |