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What evidence supports the theory of evolution by natural selection?

Describe and evaluate the lines of evidence that support evolution.

The main lines of evidence for evolution: fossils, comparative anatomy, biogeography, embryology, and molecular and biochemical data, for TCE Biology Unit 4.

Reviewed by: AI editorial process; not yet individually human-reviewed

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What this dot point is asking

Fossil evidence

Fossils are the preserved remains or traces of past organisms. Because fossils form in layers of sedimentary rock, deeper layers are generally older, allowing scientists to order them in time. The fossil record shows that life has changed over hundreds of millions of years, that many extinct species existed, and that simpler forms generally appear before more complex ones.

Transitional fossils are especially important because they show intermediate features between older and newer groups. Dating methods, including radiometric dating using the decay of isotopes, allow fossils and rock layers to be assigned absolute ages.

Comparative anatomy

Comparing the body structures of different species reveals patterns that point to common ancestry.

  • Homologous structures have the same basic underlying structure but different functions, such as the pentadactyl (five-fingered) limb seen in humans, whales, bats, and cats. The shared plan indicates descent from a common ancestor, with divergent evolution adapting the limb to different uses.
  • Analogous structures have similar functions but different underlying structure, such as the wings of insects and birds. These arise through convergent evolution, where unrelated species independently evolve similar solutions, and they do not indicate close relationship.
  • Vestigial structures are reduced features that had a function in ancestors but little or none now, such as the human appendix or the pelvic bones of whales.

Biogeography

The geographic distribution of species supports evolution. Species on isolated land masses, such as Australia's marsupials, are often unique because they evolved separately after isolation. Islands frequently have closely related species derived from a single colonising ancestor, as Darwin observed with the finches of the Galapagos. Distribution patterns match the history of continental movement and isolation rather than random placement.

Embryology and development

Many vertebrate embryos show striking similarities early in development, such as pharyngeal (gill) arches and tails, even in species that look very different as adults. These shared developmental features reflect inherited genetic instructions from a common ancestor.

Molecular and biochemical evidence

Modern biology provides some of the strongest evidence. Comparing DNA sequences, amino acid sequences of proteins, and shared biochemical pathways shows that more closely related species have more similar molecules. The near-universal genetic code and shared molecules like cytochrome c indicate that all life shares a common origin.

Evaluating the evidence

No single line of evidence proves evolution alone, but the strength of the case comes from many independent sources all pointing to the same conclusion. Fossils, anatomy, biogeography, embryology, and molecular data are consistent with each other, which is why evolution is accepted as the unifying theory of biology.

Exam-style practice questions

Practice questions written in the style of TASC exam questions on this dot point, with worked answer explainers. The year tag is the paper they imitate, not the source.

TCE 20226 marksThe forelimbs of a human, a whale, a bat and a horse all contain the same set of bones arranged in the same basic pattern, despite being used for very different functions. Name this type of structure, explain what it suggests about the ancestry of these animals, and distinguish it from an analogous structure using an example.
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A 6 mark answer names homologous structures, draws the ancestry conclusion and contrasts with analogy.

Structure type
These are homologous structures: the same underlying bone pattern modified for different uses.
What it suggests
A shared basic plan modified for different functions indicates the animals share a common ancestor that had that limb plan. The structure diverged through divergent evolution as descendants adapted to different environments.
Analogous structures (contrast)
Analogous structures have a similar function but different underlying structure and no recent common ancestor, arising by convergent evolution. Example: the wing of an insect and the wing of a bird both allow flight but are built differently and evolved independently.

Markers reward naming homologous structures, the common-ancestor/divergent-evolution conclusion, and a correct analogous example linked to convergent evolution.

TCE 20246 marksExplain how each of the following provides evidence for evolution: (a) the fossil record; (b) comparing the DNA or protein sequences of different species; (c) biogeography (the distribution of species such as marsupials in Australia). Evaluate which line of evidence is strongest and why.
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A 6 mark answer explains all three lines and makes a reasoned evaluation.

(a) Fossil record
Shows a sequence of forms over time, with transitional fossils linking groups; relative and absolute dating show simpler forms appear before more complex ones, supporting gradual change.
(b) Molecular evidence
The more similar two species' DNA or protein sequences are, the more recently they shared a common ancestor. Differences accumulate over time, so sequence comparisons reveal relationships and a molecular clock.
(c) Biogeography
Isolated regions such as Australia have unique groups (marsupials) because populations were isolated and evolved separately. Distribution reflects shared ancestry plus geographic isolation.
Evaluation
Molecular evidence is often considered strongest because it is quantitative, available for living species, and independently confirms relationships suggested by fossils and anatomy, reducing reliance on incomplete fossil preservation.

Markers reward a correct explanation of all three lines and a justified evaluation (any defensible choice with sound reasoning).

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