Quick questions on DNA sequencing and DNA profiling (HSC Biology Module 5)

4short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.

What is dNA sequencing?

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DNA sequencing determines the precise order of the nucleotide bases (A, T, G, C) along a stretch of DNA.

What is dNA profiling?

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DNA profiling (also called DNA fingerprinting) does not read the bases. It produces a pattern of fragment lengths from highly variable regions of the genome - typically short tandem repeats (STRs).

What is match depth to the command word?

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"Outline" wants brief points; "describe" wants the steps in order; "compare" wants the same features for both technologies; "evaluate" wants a justified judgement weighing strengths and limitations.

What is name the tech precisely?

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PCR (amplify), STR (the variable region), gel electrophoresis (separate by size), Sanger / ddNTP (sequencing), next-generation sequencing (parallel, fast, cheap). Precise terms lift a Band 4 answer to Band 6.

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