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Unit 2: How does inheritance impact on diversity?

Quick questions on Monohybrid crosses and test crosses: VCE Biology Unit 2

13short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.

What is setting up a monohybrid cross?
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A monohybrid cross considers one gene with two alleles.
What is the four basic monohybrid crosses (autosomal dominant pattern)?
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Using P (purple, dominant) and p (white, recessive).
What is the test cross?
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A test cross is the cross between an organism showing the dominant phenotype (unknown genotype: PP or Pp) and a homozygous recessive organism (pp).
What is worked example?
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A pea plant with round seeds (R dominant, r recessive) is found in a wild population. The grower wants to know if it is RR or Rr. They cross it with a known wrinkled pp plant (rr).
What is why monohybrid crosses matter?
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The 3:1 (and 1:1) ratios are the empirical signature of:
What is beyond Mendel?
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The same approach works with non-classical dominance:
What is 3. Heterozygous times homozygous recessive?
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This is the test cross.
What is sample-size caveat?
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With small numbers of offspring, a heterozygous parent might by chance produce all-dominant offspring just from random gamete sampling. The more offspring observed, the more confident the conclusion.
What is mixing up genotypes and phenotypes in the ratio?
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The Pp × Pp cross gives a genotype ratio of 1 PP : 2 Pp : 1 pp but a phenotype ratio of 3 purple : 1 white. Always state which you mean.
What is forgetting that a test cross requires the recessive homozygote?
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Crossing your unknown with a heterozygote (Pp) does not work cleanly because the heterozygote produces both kinds of gametes; you cannot infer the unknown's genotype from the offspring pattern.
What is treating 3:1 as exact?
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Real offspring counts vary by chance. A 3:1 cross with 100 offspring might give 78:22 or 71:29 and still be consistent with 3:1.
What is saying the test cross "creates" a homozygote?
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The test cross reveals the genotype by producing different offspring patterns from the two possibilities.
What is skipping the gametes step?
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Drawing the Punnett square without listing the gametes is the most common source of errors. Be explicit: PP produces only P gametes; Pp produces 50% P and 50% p; pp produces only p.

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